Heart preservation
Författare
Summary, in English
In study I, pre-stretched coronary arterial segments were studied in organ baths. Contractions were induced with 16 combinations of four different concentrations of potassium (16, 23, 30, and 127 mmol/L) at four different temperatures (37, 22, 15, 8 °C). Magnesium with concentrations from 0 - 20 mmol/L was used to attenuate the contraction induced with potassium (16, 23, 30, and 40 mmol/L) at 37 °C. The contractions caused by potassium can be minimized when combining hypothermia and/or elevated magnesium.
In study II, a specially designed sealed perfusion system was used to investigate the oxygen consumption of aerobically perfused cardioplegic hearts. At 37°C , each 100 g of heart tissue consumes 1.1 mL/min oxygen at rest; and the oxygen consumption is reduced to 53%, 30%, 19%, and 15%, when the temperatures were 30, 22, 15, and 8 °C , respectively.
In study III and IV, porcine hearts were perfused either 8 hours (continuously or intermittently) or 24 hours (with alternating pressures of 20 and 10 mmHg), using an oxygenated hyperkalemic perfusate containing erythrocytes, albumin, and hormones. The coronary arteries of the perfused hearts were investigated in organ baths and compared to fresh controls. The ventricular function of the 24 hours perfused hearts was similarly investigated. The intact coronary arterial endothelial function was preserved after both 8 hours and 24 hours of perfusion. The myocardial function was also fully preserved after 24 hours of perfusion.
In conclusion, with well-established perfusion preservation, the heart can be safely preserved for 24 hours.
Avdelning/ar
Publiceringsår
2018
Språk
Engelska
Publikation/Tidskrift/Serie
Lund University, Faculty of Medicine Doctoral Dissertation Series
Volym
2018
Issue
128
Fulltext
Dokumenttyp
Doktorsavhandling
Förlag
Lund University: Faculty of Medicine
Ämne
- Cardiac and Cardiovascular Systems
Status
Published
Forskningsgrupp
- Heart and Lung transplantation
Handledare
- Stig Steen
- Trygve Sjöberg
- Johan Nilsson
ISBN/ISSN/Övrigt
- ISSN: 1652-8220
- ISBN: 978-91-7619-696-0
Försvarsdatum
16 november 2018
Försvarstid
09:15
Försvarsplats
Belfragesalen, BMC D15, Klinikgatan 32 i Lund
Opponent
- Anders Jeppsson (professor)