Bacterial antibody hydrolyzing enzymes – as bacterial virulence factors and biotechnological tools
Författare
Summary, in English
This thesis highlights the importance of glycosylation for antibody function and presents different aspects and applications of antibody modifications by bacteria. We show, for the first time, activity of the IgG-specific Streptococcal endoglycosidase EndoS during Streptococcus pyogenes infection, clearly demonstrating that EndoS contributes to S. pyogenes pathogenesis and bacterial survival in the context of adaptive immunity. Further this thesis presents the use of bacterial enzymes as antibody modifying tools and their potential as binding reagents for selective antibody purification. The identification and characterization of two novel proteases, BspK and BspE exhibiting unique IgG and IgA cleavage profiles respectively, from Bdellovibrio bacteriovorus highlights the potential of using Bdellovibrio as a source for the identification of novel enzymes with biotechnological applications. Finally, I present the development of a novel method for selective antibody purification, using the inactive variants of the bacterial enzymes EndoS and EndoS2, ensuring the purification of native, correctly folded and modified antibodies.
Avdelning/ar
Publiceringsår
2019
Språk
Engelska
Publikation/Tidskrift/Serie
Lund University, Faculty of Medicine Doctoral Dissertation Series
Volym
2019
Issue
100
Fulltext
Dokumenttyp
Doktorsavhandling
Förlag
Lund University: Faculty of Medicine
Ämne
- Medical and Health Sciences
- Basic Medicine
- Microbiology in the medical area
- Immunology in the medical area
- Medical Biotechnology
Nyckelord
- Immunoglobulins
- Immunomodulation
- Antibody mediated immunity
- Glycosylation
- Bacterial virulence
- Bacterial enzymes
- Biotechnology
- streptococcus pyogenes
- Bdellovibrio bacteriovorus
Status
Published
Forskningsgrupp
- Infection and immunomodulation
Handledare
- Mattias Collin
- Oonagh Shannon
- Rolf Lood
- Andreas Nägeli
ISBN/ISSN/Övrigt
- ISSN: 1652-8220
- ISBN: 978-91-7619-829-2
Försvarsdatum
25 oktober 2019
Försvarstid
09:00
Försvarsplats
Belfragesalen, BMC D15, Klinikgatan 32 i Lund
Opponent
- Samantha J. King (PhD)